Figure 5.

Disappearance of leptospires between 3 and 6 hpi is not TLR-mediated. (A) Intracellular survival 3, 6, and 24 h post-infection of L. interrogans serovar Manilae strain MFLum1 in RAW264.7 stimulated with 1 µg/ml LPS of E coli and infected during 1 h with subsequent gentamicin protection assay. Intracellular survival was directly assessed by luminescence measurement on cell lysates diluted in EMJH. (B) Intracellular survival 3, 6, and 24 h post-infection of L. interrogans serovar Manilae strain MFLum1 in WT, TLR2 ko, TLR4 ko, and MyD88/TRIF dko BMDMs infected during 1 h with subsequent gentamicin protection assay. Intracellular survival was directly assessed by luminescence measurement on cell lysates diluted in EMJH. (A, B) Bars correspond to mean +/- SD of technical replicates (n>3). Data presented are representative of at least two independent experiments.