Figure 6.

Internalized leptospires are not degraded by ROS or NO. (A) ROS production, measured by DCF probe, in WT and TLR2/TLR4 dko BMDMs upon 6 h of infection with L. interrogans serovar Manilae strain L495. (B) NO production, measured by Griess reaction, in WT and TLR2/TLR4 dko BMDMs upon 24 h infection with L. interrogans serovar Manilae strain L495. (C) Intracellular survival 6 and 24 h post-infection of L. interrogans serovar Manilae strain MFLum1 in RAW264.7 treated with 25 µM ROS-inhibitor APDC and infected during 1 h with subsequent gentamicin protection assay. Intracellular survival was directly assessed by luminescence measurement on cell lysates diluted in EMJH. (D) Intracellular survival 24 h post-infection of L. interrogans serovar Manilae strain MFLum1 in RAW264.7 treated with 10 µM NO-inhibitor SMT and infected during 1 h with subsequent gentamicin protection assay. Intracellular survival was directly assessed by luminescence measurement on cell lysates diluted in EMJH. (A–D) Bars correspond to mean +/- SD of technical replicates (n > 3). Data presented are representative of at least two independent experiments.