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. 2022 Jul 25;19:60. doi: 10.1186/s12987-022-00359-3

Table 2.

Characterization of extracellular vesicles

Author Cell of origin Isolation procedure Size (nm) Zeta potential Positive EV markers Negative EV markers Concentration Total protein
Morad et al MDA-MB-231 Ultracentrifugation at 100000 g for 90 min at 4 °C

154.1 ± 7.0 and

158.5 ± 6.0

N.A CD9, CD63, Alix GM130 1 × 1011 particles/mL N.A
Tominaga et al MDA-MB-231-luc-D3H1 and MDA-MB-231-luc-D3H2LN Ultracentrifugation at 110,000 g for 70 min at 4 °C 100 N.A CD63, CD9 Cytochrome C 1.2 × 109 particles/mL N.A
Chen et al HEK 293 T cells Ultracentrifugation 120,000 g for 2.5 h at 4 °C 96.3 ± 5.4 and 80.3 ± 2.0 N.A CD63, CD9, CD81 N/A 3 × 108 and 6 × 108 particles/mL N.A
Matsumoto et al Human erythrocytes SEC 205.22 ± 1.79 N.A Alix CD235a (RBC) 1.76 × 109 particles/mL 0.68 ± 0.11 mg/dish
Kuroda et al SK-Mel-28 ExoQuick-TC (polymer based extraction by precipitation) and MagCapture (Affinity method for phosphatidylserine) 217.0 ± 4.5 N.A CD9,CD81,TSG101,Alix,flotillin-1 calnexin, GRP78 9 × 109 particles/mL N.A

EVs extracellular vesicles, SEC Size exclusion column, N.A. information not available