Figure 2.

ZOL/IL-2 treatment remarkably activated and expanded Vγ2Vδ2 T cells in PBL of Chinese cynomolgus macaques, and enhanced effector capabilities of Vγ2Vδ2 T cells to produce cytokines and to inhibit intracellular mycobacterial growth. (A) Representative flow cytometry histograms show that ZOL, as well as phosphoantigen (HMBPP), could activate and expand Vγ2Vδ2 T cells in PBMC from Chinese cynomolgus macaques in 7-day co-culture with IL-2. Data were gated on CD3. (B) ZOL/IL-2-expanded Vγ2Vδ2 T effector cells could produce appreciable levels of anti-TB cytokines IFN-γ and TNF-α. (C) ZOL/IL-2-expanded Vγ2Vδ2 T cells could inhibit intracellular mycobacterial (BCG) growth. Data are shown as means ± SEM in three independent experiments. ** p < 0.01, * p < 0.05.