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. 2022 Jul 21;27(1):158–166. doi: 10.1080/13510002.2022.2101832

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© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Electron micrographs of testicular tissue from the MTX group. (A) Part of Sertoli cell (Sc) with extensive cytoplasmic loss and vacuolation (V). Note also: deformed primary spermatocyte (PS) displaying perinuclear space (arrow). (B) Sertoli cell (Sc) showing a large number of primary and secondary lysosomes (Ly). (C) Early stage spermatid (Sd) appears with marked irregularities of the nucleus (N) and acrosomal cap (arrow). SP: primary spermatocyte. (D) Deleterious changes in Leydig cell (Lc) are discernible. The nucleus (N) shows contended peripheral chromatin and distended envelope. In the cytoplasm, observe the presence of many lipid vacuoles (L), sparse smooth endoplasmic reticula, and disintegrated mitochondria (M) with broken cristae. Scale bar: 5 µm (A–C), 2 µm (D).