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. 2022 Jul 21;27(1):158–166. doi: 10.1080/13510002.2022.2101832

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© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Electron micrographs of testicular tissue from the MTX + NG40 group. (A) Sertoli cell (Sc), lysosomes (arrow) in Sertoli cell cytoplasm, spermatogonium type B (Sg-B), vacuoles (V) between spermatogonia and Sertoli cell, and primary spermatocytes (PS) in normal ultrastructure. (B) Marked detachment (*) of Sertoli cell (Sc) from basal lamina (BM), vacuoles separation areas (V) between spermatogonia type B (Sg-B) and Sertoli cell (Sc), M: mitochondria, L: lipid inclusions, Ly: lysosomes, spermatozoon (Sz) in normal ultrastructure, and degenerated spermatozoa with loss of acrosome (arrow) and nuclear fragmentation (arrowhead). (C) Normal ultrastructure features of early spermatids (Sd) at different developmental stages. Arrow points to abnormal dislocation of acrosomal vesicle inside spermatid nucleus. (D) Leydig cell nucleus (N) with less condensed chromatin, and normal mitochondria (M), minimal lipid inclusions (L) and prominent smooth endoplasmic reticula (sER) in Leydig cell cytoplasm. Scale bar: 10 µm (A), 5 µm (B, C), 2 µm (D).