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. 2022 Jul 21;27(1):158–166. doi: 10.1080/13510002.2022.2101832

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© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — Electron micrographs of testicular tissue from the MTX + NG80 group. (A) Spermatogonia type B (Sg-B) and Sertoli cell (Sc) are located on basal lamina (MB), spermatocytes (SP), early spermatids (Sd) and spermatozoa (Sz) are noticed similar to the control group, and fragments of damaged spermatozoa (arrows) can also be seen. (B) Sertoli cells (Sc) with small empty spaces and vacuoles (V), and well-preserved contact between Sertoli cells and primary spermatocytes (PS). (C) Normal morphology of early spermatids (Sd). Observe malformed spermatid with vacuolated acrosomal region (red circle). (D) Normal Leydig cell nucleus (N), mitochondria (M), and smooth endoplasmic reticula (sER). Scale bar: 10 µm (A), 5 µm (B, C), 2 µm (D).