KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| PKAc | BD Biosciences | 610981; RRID: AB_398294 |
| PKARIIα | BD Biosciences | 612243; RRID: AB_399566 |
| GFP | Rockland | 600-101-215; RRID: AB_218182 |
| AKAP79 | custom rabbit polyclonal, Scott lab | VO89 |
| PKARIβ | Santa Cruz | sc-907; RRID: AB_2237411 |
| V5-tag | Thermo Fisher | R96025; RRID: AB_2556564 |
| phospho-CREB/phospho-ATF1 | CST | 9198; RRID: AB_2561044 |
| CREB | CST | 9104; RRID: AB_490881 |
| phospho-PKA substrate | CST | 9624; RRID: AB_331817 |
| StAR | CST | 8449; RRID: AB_10889737 |
| phospho-RIIβ | Santa Cruz | sc-136460; RRID: AB_10655524 |
| RIIβ | BD Biosciences | 610626; RRID: AB_397958 |
| phospho-ERK1/2 | CST | 9101; RRID: AB_331646 |
| ERK1/2 | CST | 9102; RRID: AB_330744 |
| pan ERK | BD Biosciences | 610123; RRID: AB_397529 |
| YAP/TAZ | CST | 8418; RRID: AB_10950494 |
| Bacterial and virus strains | ||
| BL21 (DE3) Competent Cells | Novagen | 70235-M |
| GC10 Competent Cells | Genesee | 42–659 |
| One Shot Stbl3 Competent Cells | Invitrogen | C737303 |
| Stable Competent Cells | NEB | C3040I |
| Biological samples | ||
| Human adrenal gland tissue | NW BioSpecimen | N/A |
| Chemicals, peptides, and recombinant proteins | ||
| Adenosine-3′,5′-cyclic monophosphate (cAMP), free acid | Biolog | A 001–500 H |
| IPTG | Sigma | I5502 |
| Glutathione Sepharose | GE Healthcare | 17075605 |
| Biotin | Sigma | B4501 |
| NeutrAvidin-HRP | Pierce | 31030 |
| Nanolink magnetic streptavidin beads | Tri-link Biotechnologies, Vector Labs | M-1002–020 |
| Endoproteinase LysC | NEB | P8109S |
| Pierce Trypsin protease, MS grade | Thermo Fisher | 90057 |
| High-Select Fe-NTA Phosphopeptide Enrichment Kit | Thermo Scientific | A32992 |
| Reprosil-Pur 120 C18-AQ, 3 mm | Dr. Maisch | R13.AQ.0001 |
| H-89 | Enzo Life Sciences | BML-EI196–0005 |
| DAPI | Life Technologies | 62248 |
| Sp-5,6-DCl-cBIMPS [5,6-Dichlorobenzimidazole riboside-3′,5′-cyclic monophosphorothioate, Sp- isomer (Sp-5,6-DCl-cBIMPS), sodium salt] | Biolog | D 014–05 |
| 6-Bnz-cAMP [N6-Benzoyladenosine-3′,5′-cyclic monophosphate (6-Bnz-cAMP), sodium salt] | Biolog | B 009–10 |
| Sp-cAMPS [Adenosine-3′,5′-cyclic monophosphorothioate, Sp-isomer (Sp-cAMPS), sodium salt | Biolog | A 003–10 S |
| Forskolin | Santa Cruz | sc-3562 |
| IBMX [3-isobutyl-1-methylxanthine] | Sigma | I7018 |
| ACTH, human | Sigma | A0423 |
| St-Ht31 disruptor peptide | Tocris | 6286 |
| St-Ht31P control peptide | Tocris | 6287 |
| Qproteome FFPE Tissue | Qiagen | 37623 |
| Critical commercial assays | ||
| Mouse/Rat ACTH ELISA kit | Abcam | ab263880 |
| Cortisol ELISA kit | Enzo Life Sciences | ADI-900-071 |
| Corticosterone ELISA kit | Enzo Life Sciences | ADI-900-097 |
| Deposited data | ||
| Autophosphorylation mass spectrometry proteomics | ProteomeXchange Consortium via the PRIDE partner repository (deposited) | 10.6019/PXD030888 |
| Proximity biotinylation mass spectrometry proteomics | MassIVE (deposited) | MSV000088654 |
| Experimental models: Cell lines | ||
| HEK293T cell line | GE Lifesciences | HCL4517 |
| U2OS PKA KO cell line | Smith et al., (2017) | N/A |
| NCI-H295R cell line | ATCC | CRL-2128 |
| ATC7L cell line | Ragazzon et al., (2006) | N/A |
| H295R-PKAcWT-miniTurbo stable cell line | This paper | N/A |
| H295R-PKAcL205R-miniTurbo stable cell line | This paper | N/A |
| H295R-PKAcW196R-miniTurbo stable cell line | This paper | N/A |
| Experimental models: Organisms/strains | ||
| Mouse: AS-Cre: Cyp11b2tm1.1(cre)Brlt/+ | Freedman et al., (2013) | N/A |
| Mouse: Floxed stop PRKACAW196R | Niswender et al., (2005) | MMRRC: 032825-MU |
| (CαR): B6.129X1-Prkacatm3Gsm/Mmmh | ||
| Mouse: C57BL/6J | The Jackson Laboratory | Strain #:000664; RRID: IMSR_JAX:000664 |
| Oligonucleotides | ||
| DNA primers | Table S1 | N/A |
| Recombinant DNA | ||
| pcDNA3.1_PKAcWT_v5 | This paper | N/A |
| pcDNA3.1_PKAcL205R_v5 | This paper | N/A |
| pcDNA3.1_PKAcW196R_V5 | This paper | N/A |
| pEYFP-N1_AKAP79 | This paper | N/A |
| pEGFP-N1_RIIα | This paper | N/A |
| pEGFP_smAKAP | This paper | N/A |
| piRFP-N1_RIα | This paper | N/A |
| piRFP-N1_RIIα | This paper | N/A |
| pPAmCherry-N1_PKAcWT | This paper | N/A |
| pPAmCherry-N1_PKAcL205R | This paper | N/A |
| pPAmCherry-N1_PKAcW196R | This paper | N/A |
| pCW57-PKAcWT-miniTurbo | This paper | N/A |
| pCW57-PKAcL205R-miniTurbo | This paper | N/A |
| pCW57-PKAcW196R-miniTurbo | This paper | N/A |
| pSMALB-PKAcWT-V5 | This paper | N/A |
| pSMALB-PKAcL205R-V5 | This paper | N/A |
| pSMALB-PKAcW196R-V5 | This paper | N/A |
| pSMALB-PKAcK72A-V5 | This paper | N/A |
| pSMALB-PKAcL205R; K72A-V5 | This paper | N/A |
| pSMALB-PKAcW196R; K72A-V5 | This paper | N/A |
| pSMALB-RIIα-PKAcWT-V5_fusion | This paper | N/A |
| pSMALB-RIIα-PKAcL205R-V5_fusion | This paper | N/A |
| pSMALB-RIIα-PKAcW196R-V5_fusion | This paper | N/A |
| pSMALB-RIIα-P2A-PKAcWT-V5 | This paper | N/A |
| pSMALB-RIIα-P2A-PKAcL205R-V5 | This paper | N/A |
| pSMALB-RIIα-P2A-PKAcW196R-V5 | This paper | N/A |
| pCW57-MCS1-P2A-MCS2 (Blast) | Adam Karpf; http://n2t.net/addgene:80921 | 80921; RRID: Addgene_80921 |
| psPAX2 | DidierTrono; http://n2t.net/addgene:12260 | 12260; RRID: Addgene_12260 |
| pMD2.G | DidierTrono; http://n2t.net/addgene:12259 | 12259; RRID: Addgene_12259 |
| pSMALB | John Dick & Peter van Galen; http://n2t.net/addgene:161786 | 161786; RRID: Addgene_161786 |
| Software and algorithms | ||
| ImageJ (FIJI) | https://imagej.net/downloads | N/A |
| GraphPad Prism 9 | www.graphpad.com | N/A |
| MaxQuant | https://www.maxquant.org/ | N/A |
| Perseus | https://www.maxquant.org/ | N/A |
| STRING database | https://string-db.org/ | N/A |
| The Gene Ontology Resource powered by PANTHER | http://geneontology.org/ | N/A |
| NetworKIN | https://kinomexplorer.info/ | N/A |