FIG. 4.

(A) A scheme of the subgroup-correlated insertion site of IS1405 with the position of each primer. The locations of IS1405b and IS1405d in 1.4- and 2.0-kb EcoRI fragments are specific to the A1 and B1 subgroup strains, respectively. (B) Agarose gel electrophoresis of PCR products from genomic DNAs of five representative A1 or B1 subgroup strains of R. solanacearum race 1 with PS-IS-F and PS-IS-RA1 or PS-IS-F and PS-IS-RB1 primer pairs, respectively. M, molecular size marker (1-kb DNA ladder, Life Technologies); sizes (base pairs) are on the left. Lane N, a negative control.