TABLE 3.
Primers used for PCRs
| Primers | Oligonucleotide sequence | Paired primer | Product size (bp) |
|---|---|---|---|
| PS-IS-F | 5′-CGCAACGCTGGATGAACCC-3′ | PS-IS-R | 1,070 |
| PS-IS-R | 5′-CAGACGATGCGAAGCCTGAC-3′ | ||
| PS-IS-Louta | 5′-GCCCGCGCTTGCGGGAGTT-3′ | PS-IS-Rout | |
| PS-IS-Rout | 5′-GCTCCGCTTCGATGACTC-3′ | ||
| PS-IS-RA1 | 5′-CACCCTAATCGGCACTAGCG-3′ | PS-IS-F | 1,181 |
| PS-IS-RB1 | 5′-CTCATGCTGACTGGCTACCC-3′ | PS-IS-F | 1,256 |
a
PS-IS-Lout and PS-IS-Rout primers were used in the inverted PCR. The size of the PCR product depends on the size of eluted DNA as described in the text.