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. 2022 Jul 22;17(1):2100685. doi: 10.1080/15592324.2022.2100685

Figure 1.

Figure 1.

The silencing of AKR2A accounts for the delayed-flowering phenotype. (a) Flowering phenotype of the AKR2A point mutant T6 and its wild-type parent BM seedlings. (b) Flowering phenotype of the Col-0 WT, the T-DNA insertion akr2a mutant, and two complementation line (AKR2A-2, AKR2A-4) seedlings. For (a) and (b), seedlings were grown under a long-day (16 h/8 h light/dark) condition. Representative images were taken at 35 d after germination. (c) PCR confirmation of the T-DNA insertion akr2a mutant. (d) Scheme of the AKR2A gene structure. Black boxes indicate the exons. The arrow indicates the position of T-DNA insertion. (e) Relative expression of AKR2A in different lines at 28 d after germination. Transcript abundance was determined by qRT-PCR. ACTIN2 served as a reference. Data are means ± SEM (n = 3, Student’s t-test, **P < .01). (f) and (g) The days from light to flowering (f) and the rosette leaf number at bolting (g) of different lines. Data are means ± SEM (n = 9, Student’s t-test, **P < .01).