Figure 3.

AKR2A interacts with FT in the cytosol. (a) Subcellular localization assay. Full-length AKR2A fused with EYFP or empty EYFP were transiently expressed in Nicotiana benthamiana mesophyll cells by infiltration. (b) BiFC detection. AKR2A was fused with the N-half of EYFP (nEYFP), and FT and CO were individually fused with the C-half of EYFP (cEYFP). Different combinations of fusion proteins as indicated were co-expressed in N. benthamiana mesophyll cells by infiltration. Images were collected 3 d after infiltration. Representative images under EYFP, chlorophyll (Chl), and bright field (Bright) channels and the merged signals are shown. Bar = 50 μm.