Table 3.
Primary antibodies used for immunohistochemistry.
| Target |
Host species |
Clone |
Dilution |
Antigen retrieval |
Supplier |
| Ki67 | Rabbit | SP6 | 1:50 | Citrate pH 6 | Thermo Fisher Scientific |
| CD31 | Rabbit | EPR17259 | 1:2000 | Citrate pH6 | Abcam |
| CAIX | Rabbit | NB100-417 | 1:1000 | Citrate pH6 | Novus Biologicals |
| CD45 | Rabbit | polyclonal | 1:1000 | Citrate pH6 | Abcam |
| CD11b | Rabbit | EPR1344 | 1:2000 or 1:4000 | Citrate pH6 | Abcam |
| Ly6G | Rat | 1A8 | 1:1000 | Citrate pH6 | BioLegend |
| CD163 | Rabbit | EPR19518 | 1:500 | Citrate pH6 | Abcam |
| CD11c | Rabbit | D1V9Y | 1:100 | Citrate pH6 | Cell Signaling Technology |
| α-SMA | Rabbit | EPR5368 | 1:2000 | Citrate pH6 | Abcam |
| FAP-α | Rabbit | polyclonal | 1:500 | Tris/EDTA pH9 | Abcam |
| CD3ε | Rabbit | EPR20752 | 1:1000 | Citrate pH6 | Abcam |
| FoxP3 | Rat | FJK-16s | 1:100 | Citrate pH6 | Thermo Fisher Scientific |
| CD19 | Rat | 6OMP31 | 1:1000 | Citrate pH6 | Thermo Fisher Scientific |
| NCR-1 | Rabbit | EPR23097-35 | 1:500 | Tris/EDTA pH9 | Abcam |
| granzyme B | Rabbit | polyclonal | 1:1000 | Citrate pH6 | Abcam |