TABLE 3.
Localization of enzymes involved in respiration in D. dehalogenansa
| Enzyme | Growth condition | Whole cells
|
Permealized cells
|
Cell extract
|
Membrane fraction
|
Soluble fraction
|
|||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Sp act (mU/mg) | Total activity (U) | Sp act (mU/mg) | Total activity (U) | Sp act (mU/mg) | Total activity (U) | Sp act (mU/mg) | Total activity (U) | Sp act (mU/mg) | Total activity (U) | ||
| LDH | Lactate + Cl-OHPA | 74 | 6.05 | 69 | 5.57 | 142 | 1.70 | 195 | 1.45 | 356 | 1.87 |
| PFO | Pyruvate + Cl-OHPA | 6 | 0.25 | 270 | 11.11 | 161 | 4.05 | 0 | 0 | 107 | 1.08 |
| FDH | Formate + Cl-OHPA | 14 | 1.25 | 108 | 9.43 | 59 | 5.26 | 190 | 1.57 | 973 | 2.04 |
| HYD | Hydrogen + Cl-OHPA | 81 | 1.52 | 110 | 2.06 | 884 | 3.04 | 206 | 0.54 | 1,726 | 0.69 |
| CPRD | Pyruvate + Cl-OHPA | 55 | 1.14 | ND | ND | 306 | 3.84 | 326 | 2.47 | 194 | 0.98 |
a
D. dehalogenans was grown with Cl-OHPA as the electron acceptor and different electron donors. Concentrated cells were permealized by the addition of 0.1% (vol/vol) CTAB. one unit is defined as the conversion of 1 μmol of substrate per min. Abbreviations: LDH, lactate dehydrogenase; PFO, pyruvate ferredoxin oxidoreductase; FDH, formate dehydrogenase; HYD, hydrogenase; CPRD, chlorophenol reductive dehalogenase; ND, not detected.