Table 1.
Primers’ sequence and qRT-PCR conditions.
| Gene Name/ Accession Number |
Primer Sequence | Annealing Temperature (°C) | Ref. | |
|---|---|---|---|---|
|
GAPDH/
NM_017008.4 |
F | AGATCCACAACGGATACATT | 52 | [67] |
| R | TCCCTCAAGATTGTCAGCAA | |||
| LC3II/NM_199500.2 | F | GAGAAGCAGCTTCCTGTTCTGG | 60 | [68] |
| R | GTGTCCGTTCACCAACAGGAAG | |||
|
Beclin-1/
NM_053739.2 |
F | GAGAAGCAGCTTCCTGTTCTGG | 60 | [69] |
| R | GTGTCCGTTCACCAACAGGAAG | |||
|
P62/
NM_175843.5 |
F | TGCCCAGACTACGACTTGTG | 56 | [68] |
| R | AGTGTCCGTGTTTCACCTTCC | |||
|
ATG5/
NM_001014250.2 |
F | GCAGATGGACAGTTGCACACAC | 56 | [70] |
| R | GAGGTGTTTCCAACATTGGCTCA | |||
|
ATG7/
NM_001012097.1 |
F | CGTTGCCCACAGCATCATCTTC | 56 | [70] |
| R | TCCCATGCCTCCTTTCTGGTTC | |||
| GAPDH, glyceraldehyde-3-phosphate dehydrogenase; LC3II, microtubule-associated protein light chain 3II; P62, ubiquitin-binding protein p62; ATG5, autophagy-related protein 5; ATG7, autophagy-related protein 7 | ||||