Table 1.
Effect of CCCP (mg/L) efflux pump inhibitor on CHX and BZK MIC (mg/L) and MBC (MFC in the case of Candida spp.) (mg/L) against Gram-negative bacteria, Gram-positive bacteria and yeasts.
| Strain | CHX MIC (MBC or MFC) | BZK MIC (MBC or MFC) | ||||||
|---|---|---|---|---|---|---|---|---|
| CCCP | CCCP | |||||||
| 0 | 1 | 2 | 4 | 0 | 1 | 2 | 4 | |
| A. baumannii ATCC 19606 | 32 (32) | 8 (8) | 4 (4) | 1 (1) | 16 (32) | 8 (16) | 8 (16) | 8 (16) |
| A. baumannii ∆adeB ATCC 19606 | 4 (4) | 2 (2) | 2 (2) | 2 (2) | 8 (8) | 4 (4) | 4 (4) | 2 (2) |
| A. baumannii ACICU | 64 (128) | 32 (64) | 8 (16) | 8 (16) | 16 (16) | 16 (16) | 16 (16) | 16 (16) |
| A. baumannii AYE | 32 (32) | 8 (8) | 4 (4) | 1 (1) | 16 (32) | 8 (16) | 8 (16) | 8 (16) |
| B. cenocepacia LMG 16654 | 64 (64) | 64 (64) | 32 (32) | 16 (16) | 64 (128) | 64 (128) | 64 (128) | 64 (128) |
| B.cepacia LMG 2161 | 32 (64) | 32 (32) | 8 (8) | 4 (4) | 32 (32) | 32 (32) | 16 (32) | 16 (32) |
| B. vietnamiensis LMG 22486 | 32 (32) | 32 (32) | 8 (8) | 4 (4) | 32 (32) | 32 (32) | 16 (32) | 16 (32) |
| B. gladioli LMG 2121 | 16 (16) | 16 (32) | 8 (16) | 4 (4) | 4 (4) | 4 (4) | 1 (1) | 1 (1) |
| B. dolosa LMG 21443 | 16 (32) | 16 (32) | 8 (16) | 4 (4) | 8 (16) | 8 (16) | 4 (8) | 4 (8) |
| B. multivorans LMG 16665 | 64 (64) | 32 (32) | 16 (16) | 8 (8) | 32 (64) | 32 (32) | 16 (16) | 8 (8) |
| E. cloacae ATCC13047 | 8 (8) | 4 (8) | 2 (4) | 1 (2) | 32 (64) | 32 (64) | 32 (64) | 32 (64) |
| E. cloacae EnC-Na-1 | 32 (32) | 16 (16) | 4 (8) | 2 (4) | 4 (4) | 4 (8) | 4 (8) | 4 (8) |
| K. aerogenes ATCC 13048 | 32 (32) | 8 (16) | 4 (4) | 2 (2) | 32 (32) | 16 (16) | 16 (16) | 16 (16) |
| K. pneumoniae ATCC 700603 | 128 (128) | 32 (32) | 8 (8) | 4 (4) | 32 (64) | 32 (64) | 32 (64) | 32 (64) |
| K. pneumoniae KP-Mo-7 | 64 (64) | 16 (16) | 4 (4) | 1 (1) | 32 (32) | 32 (32) | 32 (32) | 32 (32) |
| K. pneumoniae KP-Mo-6 | 64 (64) | 16 (16) | 4 (4) | 1 (1) | 32 (32) | 32 (32) | 32 (32) | 32 (32) |
| P. aeruginosa RP73 | 32 (32) | 32 (32) | 16 (16) | 16 (16) | 64 (128) | 64 (64) | 64 (64) | 64 (64) |
| P. aeruginosa PAO1 | 16 (16) | 16 (16) | 8 (8) | 8 (8) | 64 (128) | 64 (128) | 64 (128) | 64 (64) |
| P. aeruginosa PA14 | 16 (32) | 16 (16) | 8 (8) | 8 (8) | 64 (64) | 64 (64) | 64 (64) | 64 (64) |
| P. aeruginosa PA-Na-1 | 32 (64) | 32 (32) | 16 (16) | 16 (16) | 64 (128) | 64 (64) | 64 (64) | 64 (64) |
| S. enterica ATCC 13076 | 4 (4) | 1 (1) | 0.5 (1) | 0.5 (1) | 16 (16) | 16 (16) | 16 (16) | 4 (4) |
| S. maltophilia K279 | 32 (32) | 8 (8) | 2 (2) | 2 (2) | 16 (16) | 16 (16) | 16 (16) | 16 (16) |
| S. maltophilia LMG 10853 | 8 (8) | 4 (4) | 2 (2) | 0.5 (1) | 8 (8) | 4 (8) | 4 (8) | 4 (8) |
| S. maltophilia OBGTC20 | 64 (64) | 32 (32) | 8 (8) | 2 (2) | 32 (32) | 16 (16) | 16 (16) | 16 (16) |
| E. faecalis ATCC 29212 | 64 (64) | 16 (16) | 16 (16) | 8 (8) | 4 (8) | 4 (8) | 4 (8) | 4 (8) |
| E. faecium ATCC 6057 | 8 (8) | 4 (4) | 2 (2) | 0.5 (1) | 4 (8) | 4 (8) | 4 (8) | 2 (4) |
| S. aureus ATCC 43300 | 1 (2) | <1 (<1) | <1 (<1) | <1 (<1) | 8 (8) | <1 (<1) | <1 (<1) | <1 (<1) |
| C. albicans ATCC 10231 | 16 (16) | 16 (16) | 16 (16) | 8 (8) | 2 (2) | <1 (<1) | <1 (<1) | <1 (<1) |
| C. albicans 62033 | 16 (16) | 16 (16) | 16 (16) | 8 (8) | 4 (4) | 4 (4) | 1 (1) | 1 (1) |
| C. parapsilosis 4609 | 32 (32) | 16 (16) | 16 (16) | 4 (4) | 2 (2) | <1 (<1) | <1 (<1) | <1 (<1) |
| C. krusei 81667 | 8 (8) | 8 (8) | 8 (8) | 8 (8) | 1 (1) | <1 (<1) | <1 (<1) | <1 (<1) |
| C. glabrata 61112 | 16 (16) | 16 (16) | 16 (16) | 8 (8) | 2 (2) | <1 (<1) | <1 (<1) | <1 (<1) |
| C. tropicalis 61220 | 8 (16) | 8 (8) | 8 (8) | 8 (8) | 1 (1) | <1 (<1) | <1 (<1) | <1 (<1) |
The numbers outside and within the brackets indicate MIC and MBC or MCF values, respectively, and are expressed in mg/L. The numbers 0, 1, 2, and 4 indicate CCCP concentrations and are expressed in mg/L.