Figure 1.

Different cytoprotective activities of flavan-3-ols in the reduction of hypoxia-induced cell death in HKC-8 cells. HKC-8 cells in 24-well plates were incubated in an atmosphere containing 1% O₂ (hypoxia) or 20% O₂ (normoxia) in the absence or presence of flavan-3-ols at 100 µM for 72 h. Cell death was measured using FACS analysis. (A) Typical FACS graphs showing the positive staining populations (Annexin-V: left lower quadrant—Q3 and left upper quadrant—Q2; 7-AAD: left upper quadrant—Q2 and upper right quadrant—Q1), (B) Data are presented as mean ± standard deviation (SD) of four determinants of total cell death (Q1–3) and are a representative of three separate experiments. NS: no significant difference between medium and any of flavan-3-ols, * p < 0.01 (Medium vs. any of flavan-3-ols, one-way ANOVA), ** p < 0.001 (Catechin vs. any of epicatechin, PCB1 and PCB2, one-way ANOVA).