Figure 2.

Effect of catechin on MMP in hypoxic HKC-8 cells. HKC-8 cells in 24-well plates were incubated in the atmosphere containing 1% O₂ (hypoxia) or 20% O₂ (normoxia) in the absence or presence of 100 µM catechin for 72 h. MMP was measured using FACS analysis with JC-1 staining. (A) Typical FACS graphs showing the degree of JC-1 aggregation as indicated by the intensity (%) of red fluorescence in the gated population. (B) Data are presented as mean ± SD of three measurements. FL-2: * p < 0.0001 (Medium vs. Normoxia, two-tailed t-test), ** p = 0.0003 (Catechin vs. Medium, two-tailed t-test).