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. 2022 Jul 19;11(7):1399. doi: 10.3390/antiox11071399

Figure 7.

Figure 7

Regulation of TXNRD1 protein expression by hypoxia and flavan-3-ol treatment in HKC-8 cells. Protein samples were prepared from cells that were grown in normoxia or hypoxia in the absence (medium) or presence of 100 µM of catechin, epicatechin, PCB1 or PCB2 for 72 h. The levels of TXNRD1 protein and GAPDH were semi-quantitatively determined using Western blot analysis. (A) A typical blot of both TXNRDR1 and GAPDH protein of three separate experiments, (B) TXNRD1/GAPDH ratios in each group. * p < 0.05 (Medium vs. any of Normoxia, Catechin, Epicatechin and PCB2), ** p > 0.05 (Medium vs. PCB1) (One-way ANOVA, n = 3).