Figure 10.

Inactivation of catechin cytoprotection against hypoxia-induced cell death in HKC-8 cells. HKC-8 cells in 24-well plates were incubated in an atmosphere containing 1% O₂ (hypoxia) or 20% O₂ (normoxia) in the absence (medium) or presence of 100 µM catechin with or without TRi-1 for 72 h. Cell death was measured using FACS analysis. (A) Typical FACS graphs showing the dead cell populations (Q1–Q3), (B) Data are presented as mean ± SD of four measurements of total cell death and are a representative of three separate experiments. * One-way ANOVA: p < 0.01 (Medium vs. Catechin, Medium vs. Catechin + 1 µM TRi-1), p > 0.05 (Medium vs. Catechin + 5 µM TRi-1), and p < 0.01 (Medium vs. Catechin + 10 µM TRi-1).