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. 2022 Jun 22;11(7):1218. doi: 10.3390/antiox11071218

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Corticosterone-induced activation of microglia. (A) Immunofluorescence of GR in the hippocampus of mice. Iba1 marked microglia; GR marked glucocorticoid receptor; DAPI marked nuclei, respectively. Bar = 50 µm. (B) Immunofluorescence of GR in the BV2 cells. Bar = 50 µm. (C) Determination of the effect of CORT in BV-2 cells using MTT assay. BV-2 cell treated with CORT with indicated concentrations (n = 6). (D) TNF-α detected in the supernatant of BV-2 cells (n = 4). (E) IL-6 detected in the supernatant of BV-2 cells (n = 4). (F) Effects of CORT intervention on microglia morphology. (G) Protein expression levels of iNOS, COX2 and p-p65 detected by immunoblotting in BV-2 cells (n = 4). Differences were assessed using t-tests. The result represents the mean ± standard error of the mean. Values not sharing a common superscript letter (a,b,c) or marking (*) differ significantly at p < 0.05; those with the same letter (a,b,c) do not differ significantly (p ≥ 0.05). Ctrl: control group, CORT: BV-2 cells treated with CORT (10 μM) group.