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. 2022 Mar 29;61(22):e202202187. doi: 10.1002/anie.202202187

Figure 7.

Figure 7

A) Chemical structures of amino‐ and hydroxy‐coumarin dioxetanes. B) Chemiluminescence kinetic profiles and total light emission of 7‐amino‐ and 7‐hydroxy‐coumarin dioxetanes [10 μM] in PBS (pH 7.0) at 37 °C and chemiluminescence images of 7‐amino‐ and 7‐hydroxy‐coumarin dioxetane [50 μM] in buffer solution (pH=7.0), 10 % DMSO at 37 °C. C) Chemiluminescence kinetic profiles and total light emission of 7‐amino‐ and 7‐hydroxy‐coumarin dioxetanes [10 μM] in PBS (pH 5.0) at 37 °C and chemiluminescence images of 7‐amino‐ and 7‐hydroxy‐coumarin dioxetane [50 μM] in buffer solution (pH=5.0), 10 % DMSO at 37 °C. Chemiluminescence images of 7‐amino‐ and 7‐hydroxy‐coumarin dioxetane were taken using IVIS® Lumina, images were acquired directly after the addition of the dioxetanes, with an exposure time of 3 min.