Figure 2.

Distinctive responses of primed and overprimed Medicago truncatula seeds coping with oxidative damage along the rehydration−dehydration cycle. (a) Experimental design. Dry seeds (DS), primed (P), and overprimed (OP; occurrence of radicle protrusion during treatment) seeds were collected at 24 h of hydropriming, and subsequently during dry‐back (DB) at 2 h (PDB2, OPDB2), 4 h (PDB4, OPDB4), and 6 h (PDB6, OPDB6). (b) ROS (reactive oxygen species) levels, measured in whole seeds at the selected time points using DCFH‐DA (2′,7′‐dichlorofluorescein diacetate). (c) Expression profiles of MtSOD, MtAPX, MtTRH1, MtSPMS, and MtSPDS genes in isolated embryos. Data were analysed with two‐way ANOVA and TUKEY test. Means without common letters are significantly different (p < 0.05). R.F.U., relative fluorescence unit