Table 2.
The reaction system of the multiplex real-time qPCR and the OIE-recommended real-time qPCR.
| Multiplex Real-Time qPCR | OIE-Recommended Real-Time qPCR | ||||
|---|---|---|---|---|---|
| Reagent | Volume (μL) | Final Concentration (nM) | Reagent | Volume (μL) | Final Concentration (nM) |
| Premix Ex Taq (2×) | 10 | 1× | Premix Ex Taq (2×) | 12.5 | 1× |
| P72-F (20 μM) | 0.2 | 200 | P72-P1 (50 μM) | 1 | 2000 |
| P72-R (20 μM) | 0.2 | 200 | P72-P2 (50 μM) | 1 | 2000 |
| P72-P (20 μM) | 0.2 | 200 | P72-P (5 μM) | 1 | 200 |
| I177L-F (20 μM) | 0.1 | 100 | / | / | / |
| I177L-R (20 μM) | 0.1 | 100 | / | / | / |
| I177L-P (20 μM) | 0.1 | 100 | / | / | / |
| EP402R-F (20 μM) | 0.2 | 200 | / | / | / |
| EP402R-R (20 μM) | 0.2 | 200 | / | / | / |
| EP402R-P (20 μM) | 0.2 | 200 | / | / | / |
| MGF505-2R-F (20 μM) | 0.2 | 200 | / | / | / |
| MGF505-2R-R (20 μM) | 0.2 | 200 | / | / | / |
| MGF505-2R-P (20 μM) | 0.3 | 300 | / | / | / |
| Template | 2 | / | Template | 2.5 | / |
| Distilled water | Up to 20 | / | Distilled water | Up to 25 | / |