TABLE 2.
16S rRNA primer sequences and alignment of the primer target sequences
| Primer | Target sequences and alignmenta | GenBank accession no. | No. of mismatches |
|---|---|---|---|
| JFP (forward; 5′-AGGGTTGATAG GTTAAGAGC-3′) | 451 A-GGGTTGATAGGTTAAGA-GC 470 (Legionella pneumophila) | M59157 | |
| 417 .-A...GATA.......T.-A. 435 (Klebsiella oxytoca) | AB004754 | 7 | |
| 452 .-A...G.TG..C....T.C.. 472 (Serratia marcescens) | M59160 | 7 | |
| 409 .-A..CGATG.......T.-A. 428 (Klebsiella pneumoniae) | AJ233420 | 8 | |
| 439 .-A..CATTA.......T.-A. 458 (Enterobacter aerogenes) | U39556 | 8 | |
| 450 .A...-A.TA.A.....T.-C. 468 (Escherichia coli) | J01859 | 9 | |
| 423 .A...CA.TA.-.....T.-C. 442 (Pseudomonas aeruginosa) | AF237678 | 9 | |
| 449 .-AAAGGT..CTCC...T.-C. 467 (Alcaligenes faecalis) | AF155147 | 12 | |
| 386 .-----.A.-----.------- 390 (Methylobacterium extorquens) | AF293375 | 16 | |
| JRP (reverse; 5′-CCAACAGCTAG TTGACATCG-3′) | 818 CGATGTCAACTAGCTGTTGG 837 (Legionella pneumophila) | M59157 | |
| 815 .................... 834 (Alcaligenes faecalis) | AF155147 | 0 | |
| 802 ..............C..... 821 (Pseudomonas putida) | D84020 | 1 | |
| 802 ..............C..... 821 (Pseudomonas fluorescens) | D84013 | 1 | |
| 802 ..............C..... 821 (Chryseomonas luteola) | D84002 | 1 | |
| 728 .....AATG.C...C..... 747 (Methylobacterium extorquens) | AF293375 | 6 |
a
The control strains with the lowest numbers of mismatches as well as Methylobacterium extorquens are shown.