FIG. 2.

Determination of the concentrations of l-arginine and GSH in the medium of E. coli BL21(DE3) after prolonged cultivation according to the methods described in references 13 and 17. (A) Determined l-arginine concentrations in the cultivation medium at the indicated time points after induction. At the time of induction l-arginine was added to the culture medium to final concentrations of either 0.2 M (filled circles) or 0.4 M (open circles). (B) Concentrations of GSH (closed triangles) and total GSH (GSH+GSSG; open triangles) in the cultivation medium after addition of 5 mM GSH.