Table 3.
Qualitative and quantitative phytochemical characterization of bioactive compounds in SP4TM formulation. Results are expressed as mean ± SD of three different experiments carried out in triplicate, and the values are expressed as mg g−1 dried material (d.wt). Different lowercase letters indicate significant differences at p ≤ 0.05, as measured by one-way ANOVA followed by Tukey’s test.
| # | RT | m/z | MS/MS | λ | Compound | |||
|---|---|---|---|---|---|---|---|---|
| Common Name | Chemical Name | CAS-ID | mg g−1 d.wt | |||||
| 1 | 22.6 | 449 | 286.9 | 520 | Idaein | Cyanidin 3-O-galactoside | 27661-36-5 | 0.52 ± 0.01 m |
| 2 | 24.6 | 449 | 286.9 | 520 | Chrysanthemin | Cyanidin 3-O-glucoside | 7084-24-4 | 4.14 ± 0.12 f |
| 3 | 24.9 | 465 | 436.7; 302.7 | 260, 280 | Taxifolin-glucoside | Dihydroquercetin-3-O-glucoside | 27297-45-6 | 0.12 ± 0.01 op |
| 4 | 25.0 | 611 | 574.7; 422.7 | 260, 280 | Dihydroneohesperidin | Dihydroquercetin-7-O-diglucoside | 13241-33-3 | 2.06 ± 0.06 h |
| 5 | 25.1 | 643 | 596.6; 490.6; 318.7 | 260, 280 | Dihydromyricetin 3,4’-O-diglucoside | 0.28 ± 0.01 n | ||
| 6 | 26.4 | 463 | 301 | 520 | Peonidin 3-O-galactoside | 28148-89-2 | 1.95 ± 0.06 h | |
| 7 | 26.8 | 419 | 286.8 | 520 | Cyanidin 3-O-arabinoside | 57186-11-5 | 0.09 ± 0.01 op | |
| 8 | 26.9 | 597 | 548.9; 301.0 | 260, 280 | Quercetin 3-O-sambubioside | 83048-35-5 | 0.01 ± 0.01 p | |
| 9 | 27.5 | 609 | 342.7; 300.7 | 260, 280 | Rutin | Quercetin 3-O-rutinoside | 153-18-4 | 0.96 ± 0.02 l |
| 10 | 28.7 | 463 | 416.7; 300.7 | 260, 280 | Hyperoside | Quercetin 3-O-galactoside | 482-36-0 | 0.18 ± 0.01 no |
| 11 | 29.1 | 473 | 455.1, 427.0, 300.9 | 520 | Peonidin 3-O-arabinoside | 524943-91-7 | 0.55 ± 0.02 m | |
| 12 | 29.2 | 477 | 300.7 | 260, 280 | Miquelianin | Quercetin 3-O-glucuronide | 22688-79-5 | 0.08 ± 0.01 op |
| 13 | 30.2 | 623 | 314.7; 299.7; 270.7 | 260, 280 | Isorhamnetin-galactoside | 3′-Methylquercetin 3-O-galactoside | 6743-92-6 | 0.43 ± 0.01 m |
| 14 | 30.2 | 625 | 317.1 | 260, 280 | Myricetin 3-O-rutinoside | 41093-68-9 | 0.15 ± 0.01 op | |
| 15 | 31.0 | 477 | 356.7; 314.7 | 260, 280 | Isorhamnetin-glucoside | 3′-Methylquercetin 3-O-glucoside | 5041-82-7 | 0.15 ± 0.01 op |
| 16 | 31.9 | 463 | 416.7; 300.7 | 260, 280 | Isoquercitrin | Quercetin 3-O-glucoside | 482-35-9 | 1.47 ± 0.04 i |
| 17 | 32.5 | 463 | 300.9 | 520 | Oxycoccicyanin | Peonidin 3-O-glucoside | 68795-37-9 | 0.29 ± 0.0 1n |
| 18 | 35.5 | 301 | 256.8 | 260, 280 | Quercetin | 117-39-5 | 11.29 ± 0.43 e | |
| 19 | 36.1 | 287 | 258.9; 230.9 | 520 | Cyanidin | 13306-05-3 | 37.21 ± 1.29 b | |
| 20 | 38.5 | 303 | 284.7 | 260.28 | Taxifolin | Dihydroquercetin | 480-18-2 | 24.08 ± 0.88 d |
| 21 | 48.0 | 301 | 240.1 | 520 | Peonidin | 134-01-0 | 2.21 ± 0.07 g | |
| 22 | 48.4 | 318 | 295.4 | 260, 280 | Ampelopsin | Dihydromyricetin | 27200-12-0 | 74.23 ± 2.94 a |
| 23 | 51.9 | 317 | 297.9; 270.7 | 260, 280 | Myricetin | 529-44-2 | 30.36 ± 0.93 c | |
| 24 | 52.1 | 315 | 297 | 260, 280 | Isorhamnetin | 3′-Methylquercetin | 480-19-3 | 11.55 ± 0.35 e |
RT: Retention Time; m/z: mass-to-charge ratio; MS/MS: detected fragmentations; λ: maximum absorption wavelength; CAS-ID: Chemical Abstracts Service Identification Number.