Figure 1.

Statistics and attrition as the sequencing data are processed by the rAMPage AMP discovery pipeline. rAMPage processes RNA-seq datasets from raw reads to transcripts to putative AMPs. In this case, a putative AMP is defined as a sequence with an AMPlify score $\ge$10 for amphibians or $\ge$ 7 for insects, a length $\le$ 30 AA, and a charge $\ge$ 2. Datasets with a reference transcriptome used during assembly are indicated with an asterisk. The total number of putative AMPs (n = 1478, including 341 duplicates) are shown in purple, discovered from a total of ~53 million assembled transcripts.