Figure 8.

Animals immunized with Myhc-α 334–352 reveal the presence of autoantibodies to COA4. (A,B) Myhc-α 334–352 and SERCA2a 971–990 immunization. A/J mice were immunized with Myhc-α 334–352 or SERCA2a 971–990 in CFA emulsions or saline, and sera were collected 21 days later at termination. Diluted serum samples (1:100) were added to plates in duplicates coated with COA4, PIK3AP1, or RNase 43–56 (control). After addition of HRP-conjugated anti-mouse total Ig as the detection antibody, reactions were stopped, and OD values were then measured at 450 nm. Mean ± SEM values representing six samples per group are shown. Two-way ANOVA with Sidak’s test was used to determine significance between groups. **** p ≤ 0.001.