Table 6.

Primers sequences for detection of antimicrobial resistance genes in the Salmonella spp. isolate and multiplex PCR annealing temperature (based on [50]).

Multiplex PCR	Gene/Antibiotic	Primer Sequences 5′–3′	Annealing Temperature	Product Size (bp)
Multiplex 1	aadA streptomycin	F-GTG GAT GGC GGC CTG AAG CC R-AAT GCC CAG TCG GCA GCG	63 °C	525 bp
Multiplex 1	strA/strB streptomycin	F-ATG GTG GAC CCT AAA ACT CT R-CGT CTA GGA TCG AGA CAA AG	63 °C	893 bp
Multiplex 2	aphA1 neomycin	F-ATG GGC TCG CGA TAA TGT C R-CTC ACC GAG GCA GTT CCA T	55 °C	634 bp
Multiplex 2	aphA2 neomycin	F-GAT TGA ACA AGA TGG ATT GCR-CCA TGA TGG ATA CTT TCT CG	55 °C	347 bp
Multiplex 2	aadB gentamicin	F-GAG GAG TTG GAC TATGGA TT R-CTT CAT CGG CAT AGT AAA AG	55 °C	208 bp
Multiplex 3	tetA tetracycline	F-GGC GGT CTT CTT CAT CAT GC R-CGG CAG GCA GAG CAA GTA GA	63 °C	502 bp
Multiplex 3	tetB tetracycline	F-CGC CCA GTG CTG TTG TTG TC R-CGC GTT GAG AAG CTG AGG TG	63 °C	173 bp
Multiplex 4	sul1 sulfamethoxazole	F-CGG CGT GGG CTA CCT GAA CG R-GCC GAT CGC GTG AAG TTC CG	66 °C	433 bp
Multiplex 4	sul2 sulfamethoxazole	F-CGG CAT CGT CAA CAT AAC CT R-TGT GCG GAT GAA GTC AGC TC	66 °C	721 bp
Single PCR	floR florfenicol	F-CACGTTGAGCCTCTATATGG R-ATGCAGAAGTAGAACGCGAC	61 °C	888 bp
Multiplex 5	blaTEM ampicillin	F-TTAACTGGCGAACTACTTAC R-GTCTATTTCGTTCATCCATA	55 °C	247 bp
Multiplex 5	blaSHV ceftiofur	F-AGGATTGACTGCCTTTTTG R-ATTTGCTGATTTCGCTCG	55 °C	393 bp
Multiplex 5	blaCMY-2 ceftiofur	F-GACAGCCTCTTTCTCCACA R-TGGACACGAAGGCTACGTA	55 °C	1000 bp
Single PCR	blaPSE-1 ampicillin	F-GCAAGTAGGGCAGGCAATCA R-GAGCTAGATAGATGCTCACAA	60 °C	461 bp

Abbreviations: bp—base pairs.