Figure 2.

EndoScan technology for the identification of immunogenic regions in PlyC: (a) in silico design of peptides covering the sequences of PlyC; (b) synthesis of oligonucleotides coding for the peptides; (c) construction of a phage display library of PlyC-derived peptides; (d) a reaction of the library with specific sera; (e) immunoprecipitation with magnetic beads binding Fc fragments of antibodies; (f) amplification by PCR reaction; (g) next-generation sequencing (NGS) (modified from Xu et al. [14]).