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. 2022 Jul 18;11(7):966. doi: 10.3390/antibiotics11070966

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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IgG reactivity against PlyC and its subunits, PlyCA and PlyCB, in human sera. Blood sera from healthy volunteers (n = 56) were tested against recombinant PlyC, PlyCA, and PlyCB by ELISA. (a) Levels of antibodies as ELISA signals; the mean value was presented as the vertical line, 25th and 75th percentile were presented as the box, whiskers designate outliers cut-off, points (triangles or circles) represent outliers in each group, * indicates a p-value of <0.0001, and all five outliers that demonstrated the highest levels of anti-PlyCB IgG (circles) and also the highest levels of anti-PlyC IgG’s (triangles). (b) Correlation (blue line) between levels of antibodies specific to PlyC and antibodies specific to PlyCA (insignificant). (c) Correlation (blue line) between levels of antibodies specific to PlyC and antibodies specific to PlyCB. The correlation between levels of antibodies specific for PlyC and PlyCB was statistically significant (p < 0.0001).