FIG. 3.

Expression of the Mn(II)-oxidizing protein(s) during the time course shown in Fig. 2. Duplicate gels of cell extracts collected from time points throughout the growth curve were stained for total protein (left) or assayed for in-gel Mn(II)-oxidizing activity (right). Arrows correspond to the Coomassie-stained proteins responsible for Mn(II) oxidation. The last sample in both gels (lane 160*) was heat-treated at 65°C for 5 min prior to electrophoresis.