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In-gel oxidation of both Mn(II) and the organic substrate, p-phenylenediamine, by the 150- and 250-kDa proteins of strain SD-21. Gels were incubated in either Coomassie blue (lane 1), Mn(II) buffer (lane 2), or HEPES-buffered p-phenylenediamine (lane 3). The arrows highlight the two bands present in all three lanes.
