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. 2022 Jun 28;10(7):1525. doi: 10.3390/biomedicines10071525

Figure 3.

Figure 3

Inhibition effect of NO on influenza virus. (A,D) H1N1, (B,E) H3N2, (C,F) Influenza B. Left Graphics: Confluent cells were infected with the indicated virus at 100 PFU/well and then treated with 160 ppm NO for up to 2.5 h (triangles) or medical air as control (squares). Virus infectivity was assessed by plaque reduction assay. Right Graphics: Virions (1000 PFUs) suspended in normal saline were treated prior to infection with 80 or 160 ppm NO for 10–120 min (triangles) or medical air as control (squares). At the end of the treatment, the suspensions were used to infect a monolayer of confluent cells. Infectivity was assessed by plaque-reduction assay. * or ** p < 0.05. Reproduced with permission [65].