FIG. 3.

Inhibitory activity in L. innocua 743 and insertion mutants. (A) Direct antagonism plate test demonstrating production of inhibitory activity in L. innocua 743 and each transposon insertion mutant. (B) Inhibitory activity of E. coliTOP10 recombinants as determined by direct antagonism testing. Spots: 1, pCR2.1-TOPO; 2, pCR2.1-32; 3, pCR2.1-48; 4, pCR2.1-83; 5, pCR2.1-100; 6, pCR2.1-228. The cloned amplicons were each generated using the P2 and BacR primers. In each case the indicator organism using in the agar overlay was L. ivanovii 27.