Figure 4.

Effects of LCN2 deletion on microglial activation and hippocampal TREM2 expression in HFD/STZ-induced diabetic mice: (A,B) Western blot analysis and quantification of LCN2, TREM2, and NF-κBp65 in the hippocampus; β-actin and p84 were used as loading controls for total protein and nuclear protein, respectively; (C) representative immunofluorescence images of TREM2 (red) and Iba-1 (green) in hippocampal sections; DAPI (blue) was used to stain nuclei; scale bar = 10 µm; (D) quantification of co-localized TREM2 and Iba-1-immunostained cells in the images. CTL: control; DM: diabetic mice. Data are presented as the mean ± SEM. The indicated p-values represent a two-way ANOVA, followed by Tukey’s post hoc test. * p  <  0.05 vs. WT CTL. ^† p < 0.05 vs. WT DM.