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. 2022 Jul 13;10(7):1692. doi: 10.3390/biomedicines10071692

Figure 2.

Figure 2

Analysis of apoptotic and necrotic fractions of PaTu-8988t, Colo357, and Panc89 cells, transfected with miR506-3p vs. NC1, by flow cytometry. (A) Original data from the cell line PaTu-8988t with apoptosis (A), necrosis (N), and debris (D) indicated. (B) Quantitation of apoptotic and necrotic cell fractions in PaTu-8988t, Colo357, and Panc89 cell samples. (C) Caspase-3/7 activation in PaTu-8988t (72 h), Colo357 (120 h) and Panc89 (72 h) cells, upon transfection with miR506-3p vs. NC1. (D) Western blot analyses of alterations in various apoptosis-related genes in PaTu-8988t (72 h) and Colo357 (120 h) cell lysates after miR506-3p vs. NC1 transfection. Expression levels of proteins related to antiapoptosis (bcl2, bcl-xL, p53, IRS-1) and survivin in PaTu-8988t (left) and Colo357 cells (right). * p < 0.05, ** p < 0.01 and *** p < 0.001.