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. 2022 Jul 13;10(7):1692. doi: 10.3390/biomedicines10071692

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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(A) Induction of senescence after transfection of PaTu-8988t (upper panel) or Colo357 cells (lower panel) with miR506-3p (red) vs. NC1 (gray), as determined by flow cytometry. (B) Confocal microscopy of PaTu-8988t cells transfected with miR506-3p (lower) vs. NC1 (upper panel) and stained with the senescence detection kit (green) and DAPI (nuclei; blue). (C) Flow cytometry-based determination of accumulation efficacies of the dye MitoView 633 in the mitochondrial membrane after transfection with miR-506-3p (green) vs. NC1 (gray) in PaTu-8988t (upper panel) or Colo357 cells (lower panel). (D) Confocal microscopy of PaTu-8988t cells transfected with miR506-3p (lower) vs. NC1 (upper panel) and stained with DAPI (left) and MitoView 633 (red; center panels). Boxes: areas selected for higher magnification; arrows: elongated mitochondria. Scale bars: 50 µm, higher mitochondria magnification: 15 µm.