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. 2022 Jul 13;10(7):1692. doi: 10.3390/biomedicines10071692

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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(A) Analysis of cell cycle distribution in PaTu-8988t (upper) cells and Colo357 cells (lower panel) after miR506-3p (red) vs. NC1 transfection (gray). (B) Quantitation of cell cycle distribution in PaTu-8988t (left) cells and Colo357 cells (right). (C) Histograms of cell cycle distribution upon transfection of PaTu-8988t (upper) cells and Colo357 cells as above + treatment with nocodazole for inducing a G2/M arrest 52 after transfection and 20 h before analysis. (D) Relative mRNA levels of cell-cycle-related cyclins CDK1, CDK2, CDK4, and CDK6 after miR506-3p vs. NC1 transfection. (E) Western blot analyses of cell-cycle-related proteins in PaTu-8988t (left) and Colo357 cell lysates (right) after miR506-3p vs. NC1 transfection. * p < 0.05, ** p < 0.01 and *** p < 0.001.