Figure 2.

Measurement of Wnt5a expression in wound by in-situ hybridization and real-time quantitative PCR. Wnt5a expression is indicated in red in the tissue. Wnt5a was specifically expressed in the dermal layer around the epidermal-dermal junction and around hair follicles. (A–C) Weakly magnified images of the region around the wound. (D–F) Strongly magnified images of the wound margin. (G–I) Observed images of the normal area. (J) Decreased expression of Wnt5a at the wound site on E13. * p < 0.05. (A–C) Scale bar = 200 μm. (D–I) Scale bar = 100 μm.