Figure 3.

Measurement of Wnt5b expression in the wound by in-situ hybridization and real-time quantitative PCR. Wnt5a expression is indicated in red in the tissue. Wnt5b expression was confirmed in the dermal layer. Expression was increased in E13, but in E15 and E17, Wnt5b expression in the wound area was decreased compared to that in the normal areas. (A–C) Weakly magnified images of the region around the wound. (D–F) Strongly magnified images of the wound margin. (G–I) Observed images of the normal area. (J) Expression at the wound site was decreased on E15 and E17. * p < 0.05. (A–C) Scale bar = 200 μm. (D–I) Scale bar = 100 μm.