TABLE 1.

Retention volumes and absorption maxima in HPLC analyses of products formed by E. coli(pSTE7) and E. coli(pSTE44) cells from chlorobenzenes and di- and trichlorotoluenes

Substrate	Proposed product(s)	[MeOH] (%)a	RV (ml)b	λmax (nm)c	Growth substrated
Chlorobenzene	3-Chloro-cis-1,2-dihydroxy-1,2-dihydrocyclohexa-3,5-dienea	36	3.4	272	+
	4-Chloro-cis-1,2-dihydroxy-1,2-dihydrocyclohexa-3,5-dienee	36	4.4	265
	3-Chloro-1,2-dihydroxybenzenef	58	2.6	210
	4-Chloro-1,2-dihydroxybenzenef	58	2.9	210
1,2-Dichlorobenzene	3,4-Dichloro-cis-1,2-dihydroxy-1,2-dihydrocyclohexa-3,5-dienee	36	6.8	272	+
	3,4-Dichloro-1,2-dihydroxybenzenef	58	4.2	210
1,3-Dichlorobenzene	NDg
	3,5-Dichloro-1,2-dihydroxybenzenef	58	5.3	210	+
1,4-Dichlorobenzene	3,6-Dichloro-cis-1,2-dihydroxy-1,2-dihydrocyclohexa-3,5-dieneef	36	4.1	283	+
	3,6-Dichloro-1,2-dihydroxybenzenee	58	4.0	210
1,2,3-Trichlorobenzene	3,4,5-Trichloro-cis-1,2-dihydroxy-1,2-dihydrocyclohexa-3,5-dienee	72	2.3	282	−
	3,4,5-Trichloro-1,2-dihydroxybenzenef	58	8.6	210
1,2,4-Trichlorobenzene	3,4,6-Trichloro-cis-1,2-dihydroxy-1,2-dihydrocyclohexa-3,5-dieneef	58	3.4	288	+
	3,4,6-Trichloro-1,2-dihydroxybenzenef	58	8.3	210
1,2,4,5-Tetrachlorobenzene	3,4,6-Trichloro-1,2-dihydroxybenzeneef	58	8.3	210	+
23DCT	2,3-Dichlorobenzylalcoholef	60	5.4	201	−
24DCT	2,4-Dichlorobenzylalcoholef	60	6.2	201	+
	4,6-Dichloro-3-methyl-cis-1,2-dihydroxy-1,2-dihydrocyclohexa-3,5-dienee	60	3.7	278
	4,6-Dichloro-3-methyl-1,2-dihydroxybenzenef	60	7.5	205
25DCT	2,5-Dichlorobenzylalcoholef	60	6.1	201	+
	3,6-Dichloro-4-methyl-cis-1,2-dihydroxy-1,2-dihydrocyclohexa-3,5-dienea	60	2.9	280
	3,6-Dichloro-4-methyl-1,2-dihydroxybenzenef	60	6.0	204
26DCT	2,6-Dichlorobenzylalcoholef	60	3.9	203	−
	3,5-Dichloro-4-methyl-cis-1,2-dihydroxy-1,2-dihydrocyclohexa-3,5.dienee	60	3.7	275
	3,5-Dichloro-4-methyl-1,2-dihydroxybenzenef	60	6.3	203
34DCT	3,5-Dichloro-6-methyl-cis-1,2-dihydroxy-1,2-dihydrocyclohexa-3,5-dienee	60	3.5	276	+
	3,4-Dichloro-6-methyl-1,2-dihydroxybenzenef	60	6.6	204
35DCT	3,5-Dichlorobenzylalcoholef	60	7.4	203	−
245DCT	2,4,5-Trichlorobenzylalcoholef	60	12.1	205	−

^a[MeOH], percentage of methanol used in the solvent system. 

^bRV, retention volume. 

^cλ_max, wavelength of maximal absorption. 

^d+, used as a growth substrate; −, not used as a growth substrate (27). 

^eProposed product derived by transformation of the indicated substrate with E. coli-(pSTE7) producing TecA dioxygenase. 

^fProposed product derived by transformation of the indicated substrate by E. coli(pSTE44) simultaneously producing TecA tetrachlorobenzene dioxygenase and TecB dehydrogenase. 

^gND, not done for E. coli(pSTE44).