Table 1. Effect trends of ASY1, ASY3 and REC8 on several key meiotic traits.
| Trait | ASY1* TTT / TDT |
ASY3* TTT / TTD |
ASY1+3* TTT / TDD |
REC8 TTT / DTT |
All 3 TTT / DDD |
Comments |
|---|---|---|---|---|---|---|
| Rods (Tet>Dip) |
+60% | (+22%) | +50% | (-10%) | = | ASY1 (and less so ASY3) increase rods, but, it appears, only when REC8 = T; could be antagonistic? |
| MV (Neo>Est) |
-29% | (-17%) | (-24%) | = | -32% |
Only axis genes, primarily ASY1 |
| UV (Neo>Est) |
-71% | +86% | Antagonistic effect | |||
| HEI10dist (Est>Neo) |
-18% | (+22%) | Antagonistic effect | |||
| SClgth (Est<Neo) |
-11% | (-3.5%) | (-4.6%) | (-6%) | (-5%) | T alleles of all 3 correlate with shorter SC |
| SPS# (Est<Neo) |
-27% | (-16%) | (-11%) | (-24%) | -40% | All 3 contribute additively to reducing SPS# |
Table notes: Effects of ASY1, ASY3 and REC8, or all three (column headings) on several key meiotic traits that differ between diploids and tetraploids (rods), or between neotetraploids and evolved tetraploids (all other traits) as shown below each trait name, where “Dip” = Diploid, “Tet” = Evolved Tetraploid, “Neo” = neo-tetraploid, “Est” = established tetraploid. “MV” = multivalent frequency, “UV” = univalent frequency, “HEI10dist” = single HEI10 focus distalisation, “SClgth” = SC length, “SPS#” = SPS site number. Homozygous genotypes for all three compared to yield single gene effects are given in headers for all three genes in each comparison as e.g. TTT vs DDD, showing REC8, ASY1, ASY3 in turn. Statistically significant differences are given in bold, and non-significant, but clearly trending ones in parentheses (as mentioned in the text, “trend” indicates a clear, but statistically not quite significant difference, e.g. p > 0.05, but near or below p = 0.1). Trend “direction” is given by a plus or minus with respect to the “T” (tetraploid) allele. In other words, “-”means that homozygotes for the T allele have a lower trait value relative to homozygotes for the D allele. “=“ indicates trait values for D and T are about equal. * Indicates that the left three columns are data trends from a separate, previously published experiment on ASY1 and ASY3 where REC8 was T [15].