Table 4.
Enzymatic properties of nattokinase from different microorganisms.
| Strain | MW (kDa) |
Optimum pH | Optimum Temperature (°C) |
Substrate Specificity | Specific Inhibitor | Metal Agonist | Metal Inhibitor | References |
|---|---|---|---|---|---|---|---|---|
| Bacillus subtilis Natto B-12 | 29 | 8 | 40 | - | - | Zn2+ | Al3+, Fe3+ | [17] |
| Bacillus subtilis BK-17 | 31 | - | - | H-D-Val-Leu-Lys-pNA | Chymostatin | - | Zn2+ | [67] |
| Bacillus sp. strain CK 11-4 | 28.2 | 10.5 | 70 | H-D-Val-Leu-Lys-rNA | PMSF | - | - | [23] |
| Bacillus sp. strain DJ-4 | 29 | 10 | 40 | - | PMSF | - | Cu2+, Zn2+ | [26] |
| Bacillus subtilis IMR-NK1 | 31.5 | 7.8 | 55 | N-succinyl-Ala-Ala-Pro-Phe-pNA | PMSF, NBS | - | - | [68] |
| Bacillus amyloliquefaciens DC-4 | 28 | 9 | 48 | N-succinyl-Ala-Ala-Pro-Phe-pNA | PMSF | - | - | [19] |
| Bacillus subtilis DC33 | 30 | 8 | 55 | N-Succinyl-Ala-Ala-Pro-Phe-pNA | PMSF, SBTI | - | Cu2+, Fe2+, Sn2+, Ag+, Ti2+ | [21] |
| Bacillus subtilis QK02 | 28 | 8.5 | 55 | - | PMSF | - | - | [27] |
| Bacillus subtilis LD-8547 | 30 | 8 | 50 | - | PMSF | Al3+, Zn2+ | Mn2+, Ba2+ | [20] |
| Bacillus subtilis TKU007 | 28–30 | 7 | 50 | - | - | - | - | [30] |
| Bacillus subtilis RJAS19 | 35 | 9.5 | 65 | - | - | - | - | [28] |
| Bacillus subtilis YJ1 | 27.5 | 8.5 | 50 | - | PMSF | Co2+, Ba2+ | Fe3+, Hg2+, Cu2+, Zn2+ | [29] |
| Bacillus subtilis TKU007 | 28–30 | 8 | 50 | - | PMSF | - | Ca2+, Cu2+ | [30] |
| Pseudomonas sp. TKU015 | 21–24 | 7 | 50 | N-benzoyl-Val-Gly-Arg-pNA and D-Val-Leu-Lys-pNA | PMSF | Fe2+ | Fe2+ | [16] |
| Pseudomonas aeruginosa CMSS | 27 | 5 | 45 | - | PMSF | Mn2+ | Ba2+, Mg2+ | [15] |
| Bacillus ereus VITSDVM3 | - | - | - | - | - | - | Cu2+, Ca2+ | [25] |
| Bacillus subtilis WRL101 | 29 | 11 | 47 | Met-Suc-Arg-Pro-Tyr-pNA(S-2586) | PMSF | Mg2+ | Zn2+, Cu2+ | [24] |
| Bacillus subtilis VTCC-DVN-12-01 | 27.7 | 9 | 65 | - | SDS | Mg2+ | - | [65] |
| Bacillus subtilis JNFE0126 | - | 7 | 40 | - | - | - | - | [18] |
| Bacillus subtilis VITMS 2 | 29 | 7 | 30 | N-Succinyl-Ala-Ala-Pro-Phe-pNA (S7338) | PMSF, EDTA | Ca2+, Zn2+, Co3+ | Hg2+, Cu2+ | [34] |
| Bacillus subtilis K2 | 26 | - | - | - | - | - | - | [35] |
| Bacillus subtilis ICTF-1 | 28 | 9 | 50 | - | PMSF | Zn2+, Fe2+, Hg2+ | - | [36] |
PMSF, Phenylmethylsulfonyl fluoride; NBS, N-Bromosuccinimide; SBTI, Trypsin inhibitor; SDS, Sodium dodecyl sulfate; EDTA, Ethylene diamine tetraacetic acid.