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. 2022 Jul 7;12(7):893. doi: 10.3390/brainsci12070893

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Flow-cytometric analysis of myeloid cells in primary implanted tumors and tumors 1- and 2-weeks post-resection in the GL261-C57BL/6 mouse glioma implantation model. (A–C) The left column contains forward scatter (FSC) vs. CD45 plots demonstrating CD45 populations: low (R3) and high (R4). The middle column contains CD11b⁺ vs. C11c⁺ events plotted from CD45^high and CD45^low populations. Graphs showing proportional composition of cellular subtypes are shown in the right column. (D) Calculation of CD45^high and CD45^low populations of microglia/macrophages purified from primary implanted tumors and tumors 1- and 2-weeks post-resection. Mean + S.D., and significant differences from the primary implanted tumor (* p < 0.005) are shown (N = 6).