Table 3.
miRNAs significantly associated with maternal BMI at 1st trimester of pregnancy in Gen3G cohort and fully replicated in 3D cohort.
| Gen3G | 3D | ||||||
|---|---|---|---|---|---|---|---|
| miRNA | DESeq2 Normalized Read Count (Mean ± SD) | Fold Change * | Unadjusted p-Value | FDR-Adjusted q-Value | DESeq2 Normalized Read Count (Mean ± SD) | Fold Change * | Unadjusted p-Value |
| hsa-miR-1323 a | 146.39 ± 230.60 | 0.957 | 2.79 × 10−10 | 9.60 × 10−8 | 676.45 ± 581.88 | 0.966 | 2.56 × 10−5 |
| hsa-miR-516b-5p a | 101.50 ± 150.35 | 0.958 | 1.79 × 10−10 | 9.60 × 10−8 | 327.56 ± 247.26 | 0.966 | 2.92 × 10−5 |
| hsa-miR-525-5p a | 9.07 ± 15.31 | 0.952 | 1.63 × 10−9 | 4.21 × 10−7 | 79.89 ± 71.16 | 0.982 | 0.04471 |
| hsa-miR-516a-5p a | 31.25 ± 53.81 | 0.959 | 4.55 × 10−9 | 7.83 × 10−7 | 150.92 ± 135.76 | 0.974 | 0.00141 |
| hsa-miR-518e-5p a | 43.96 ± 62.52 | 0.962 | 8.92 × 10−9 | 1.22 × 10−6 | 99.07 ± 88.52 | 0.981 | 0.02118 |
| hsa-miR-520a-3p a | 86.75 ± 132.37 | 0.960 | 9.41 × 10−9 | 1.22 × 10−6 | 220.91 ± 243.90 | 0.982 | 0.04009 |
| hsa-miR-518e-3p a | 7.99 ± 14.22 | 0.956 | 2.02 × 10−8 | 2.09 × 10−6 | 29.60 ± 25.75 | 0.971 | 0.00189 |
| hsa-miR-512-3p a | 287.04 ± 575.48 | 0.963 | 3.63 × 10−8 | 3.41 × 10−6 | 771.74 ± 982.11 | 0.982 | 0.02747 |
| hsa-miR-1283 a | 57.10 ± 87.82 | 0.962 | 4.17 × 10−8 | 3.59 × 10−6 | 203.52 ± 173.84 | 0.977 | 0.00635 |
| hsa-miR-517a-3p a | 20.19 ± 37.78 | 0.960 | 5.17 × 10−8 | 4.11 × 10−6 | 76.56 ± 73.88 | 0.980 | 0.01599 |
| hsa-miR-526b-5p a | 17.12 ± 25.90 | 0.962 | 2.19 × 10−7 | 1.62 × 10−5 | 39.62 ± 34.83 | 0.977 | 0.01056 |
| hsa-miR-517-5p a | 16.90 ± 29.75 | 0.962 | 9.56 × 10−7 | 6.17 × 10−5 | 70.67 ± 67.45 | 0.981 | 0.02191 |
| hsa-miR-519c-3p a | 11.08 ± 20.08 | 0.965 | 5.11 × 10−6 | 0.00026 | 36.44 ± 32.83 | 0.966 | 0.00034 |
| hsa-miR-519d-5p a | 6.12 ± 9.51 | 0.961 | 1.53 × 10−5 | 0.00063 | 10.75 ± 11.77 | 0.969 | 0.01205 |
| hsa-miR-515-5p a | 10.50 ± 19.12 | 0.967 | 1.50 × 10−5 | 0.00063 | 26.70 ± 26.08 | 0.968 | 0.00076 |
| hsa-miR-27b-3p | 29,699.76 ± 20,347.78 | 1.008 | 4.98 × 10−5 | 0.00172 | 55,214.27 ± 41,098.16 | 1.011 | 0.00110 |
| hsa-miR-885-5p | 13.50 ± 17.92 | 1.036 | 4.98 × 10−5 | 0.00172 | 40.63 ± 78.98 | 1.034 | 0.00588 |
| hsa-miR-520a-5p a | 3.06 ± 5.66 | 0.963 | 0.00032 | 0.00830 | 27.63 ± 27.09 | 0.965 | 0.00136 |
| hsa-miR-375 | 1938.28 ± 3939.32 | 0.975 | 0.00040 | 0.00986 | 2900.08 ± 2878.58 | 0.982 | 0.03889 |
| hsa-miR-520d-3p a | 9.80 ± 15.84 | 0.972 | 0.00091 | 0.01877 | 10.77 ± 13.37 | 0.966 | 0.00638 |
| hsa-miR-592 | 1.64 ± 3.00 | 1.042 | 0.00162 | 0.03103 | 2.43 ± 4.89 | 1.058 | 0.00400 |
| hsa-miR-21-5p | 85,256.17 ± 61,108.55 | 1.005 | 0.00224 | 0.03811 | 82,095.57 ± 63,195.51 | 1.008 | 0.02738 |
Models adjusted for sequencing run and lane, gestational age, maternal age at the first trimester of pregnancy, and parity. * Fold changes represent the change in miRNA abundance for each increase of one unit of maternal BMI at 1st trimester of pregnancy. a miRNAs from C19MC. Abbreviations: 3D: Design, Develop, Discover birth cohort; FDR: false discovery rate; Gen3G: Genetics of Glucose regulation in Gestation and Growth birth cohort; SD: standard deviation.