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. 2022 Jul 6;12(7):951. doi: 10.3390/biom12070951

Figure 2.

Figure 2

Focused ultrasound and IgG2a immunoreactivity (IR). (A) Representative DAB staining (anti mouse-IgG2a) for the left and right hemispheres in the unilateral FUS cohort. (B) Triple immunofluorescence labelling for Iba1, S97 (general Aβ) and anti-IgG2a indicating presence of antibody with Aβ plaques on the FUS side (right hemisphere). Unilateral FUS on the right hemisphere showed (C) increased anti-IgG2a IR in the cortex at 4 h (p < 0.05) and 72 h (p < 0.05) after 07/2a treatment and (D) no significant changes in the hippocampus; paired-t-test. (E,F) Cortical and hippocampal anti-IgG2a IR in the bilateral FUS cohort at 4 h and 72 h post-treatment. Arrowheads indicating IgG2a IR with some possible staining around plaques. Increased anti-IgG2a IR was found in the (G) cortex and (H) hippocampi after combination treatment at both time points, however, a significant increase was found in the cortex at 4 h. Black dots represent data from individual mice. Two-Way ANOVA, with Bonferroni’s multiple comparison test *, p < 0.05. Scale bar, (A) = 200µm and (B,E) = 20 µm.