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Western blot for S-protein expression. Lanes 1–5: recombinant SARS-CoV-2 S-protein RBD (0.25 pmol, 0.5 pmol, 1 pmol, 2 pmol, 4 pmol); Lanes 6–8: K562 lysates (control, 12 h, 3 h); Lanes 9–11: PBMCp lysates (control, 24 h, 3 h); Lanes 12–14: HL-60 lysates (control, 12 h, 3 h). No difference in antibody binding was seen between vaccine-treated cells and negative controls at the expected molecular weights for full-length S-protein or fragments.
