Figure 2.
Effect of platelet supernatant on the growth of E. coli strains. Bacteria (20 μL, 3 × 108 CFU) were added to filtered supernatant (180 μL) and incubated together at 37 °C for four hours while being rotated. (A) Supernatant effect of E. coli platelet-sensitive strains BL21DE3 and ATCC11303 (n = 5). (B) Supernatant effect of E. coli platelet-resistant strains K12, ATCC 25922 and Q1065 (n = 5). Ctr−: bacteria alone (blue column); Plt J53: bacteria incubated with supernatant of platelets stimulated by J53 (dark grey column); Ctr+: bacteria incubated with supernatant of platelets treated by TRAP (light grey column). Bars represent Mean with SD. *: significant increase; #: significant decrease, ns: non-significant. Significant differences between the two groups were determined by Bonferroni test preceded by two-way ANOVA. * and #: p < 0.05, ** and ##: p < 0.01, ns: p > 0.05.